Glass capillaries were heated and pulled by a microcapillary puller p, sutter. Both the liquid jet and the gas stream cross the orifice of a plate located in front of the capillary at a distance h. The double emulsion device was tested at a variety of flow conditions to demonstrate the formation of varied size, monodisperse double emulsions. To achieve this, a classic capillary microfluidic device is firstly designed to. No other driving force is required, apart from traditional pumping, a key difference with other comparable technologies, such as electrospray where an electric field is needed. Double emulsion generation using a polydimethylsiloxane. However, it is difficult to generate monodisperse doubleemulsion drops with ultrathin shells using this. The droplets generated by the device are monodisperse with d 1. The system consists of polydimethylsiloxane droplets of varying sizes and viscosities dispersed in a polyisobutylene matrix. Effect of confinement and viscosity ratio on the dynamics of. Monodisperse double emulsions generated from a microcapillary.
While tracing out spatial paths with an injection tip, the granular gel fluidizes at the point of injection and then rapidly solidifies, trapping injected material in place. In the reservoir, the curvature decreases as the tongue grows. Monodisperse double emulsions generated from a microcapillary device. Jan 15, 20 then the dispersed phase can be injected by hand, also using a pipette. This paper reports a new method that controls both the size and the number of double emulsion droplets through a microcapillary technique. Highly uniform emulsion droplets can be generated by.
The meniscus is stretched until a thin jet tapers from its tip due to the action of a gas stream driven by a pressure drop. A 3d printed microcapillary assembly for facile double emulsion generation. A modular microfluidic device via multimaterial 3d printing for emulsion generation. We report a parallelized capillary microfluidic device for enhanced production rate of monodisperse polymersomes. To form www double emulsion, we use monodisperse ww droplets as templates, and. During generation of the doubleemulsion drops, the. Allaqueous coreshell droplets have recently been generated through a process of spontaneous phase separation. Four reversible and reconfigurable structures for threephase emulsions. Collective generation of milliemulsions by stepemulsification. Recently, microfluidic emulsification devices have attracted much attention due to their unprecedented. Main journal of colloid and interface science monodisperse alginate microcapsules with oil core generated from a microfluidic device journal of colloid and interface science 2010 vol. Double emulsions are dispersions of a third liquid inside the droplets of the first emulsion. By associating both water and oilsoluble surfactants in various concentrations, we produce wow double emulsions.
Microfluidic devices can generate double emulsions in a single step, allowing precision control of. Common types of emulsions include oilinwater ow, such as milk, and water in oil wo, like butter. The effect of the degree of confinement and viscosity ratio is systematically investigated. Although double emulsions are potentially of commercial value, traditional fabrication by means of two emulsification steps leads to very illcontrolled structuring. Shihhao huang 1,2, weiheong tan 1, fangang tseng 2,3 and shoji takeuchi 1,4. Using a microcapillary device, we fabricated double emulsions that contained a single internal droplet in a core shell geometry. The output is a dispersed liquid or gas, frequently in the form of a fine aerosol or an emulsion. This contrasts with the case of a sloped reservoir for which the depth of. Flow focusing may be applied in the food, medicine, pharmaceutical, cosmetic, photographic and environmental industry, among other potential uses.
Simple and inexpensive microfluidic devices for the. Double emulsion production in glass capillary microfluidic device. These double emulsions can encapsulate molecules in the inner aqueous phase with essentially 100% ef. Monodisperse double emulsions generated from a microcapillary device as utada, e lorenceau, dr link, pd kaplan, ha stone, da weitz science 308 5721, 537541, 2005. Flow focusing is a technology whose aim is the production of drops or bubbles by straightforward hydrodynamic means.
Controllable monodisperse multiple emulsions from softmatter. However, there has been no report on the microfluidic device for the preparation of wow emulsions with an ultrathin, nanometerthick, oil phase layer. Writing in the granular gel medium science advances. Previously, methods to produce double emulsions resulted in a high degree of variability, making overall yield of useful double emulsions quite small. Monodisperse double emulsions generated from a microcapillary device a. Four reversible and reconfigurable structures for threephase.
The fluid then spontaneously breaks up into monodisperse drops as it passes the nozzles. Some stability criteria for double emulsions langmuir. In this paper, the authors present a mechanism by which to produce monodisperse double emulsions with a high degree of control. Controlled formulation of monodisperse double emulsions in. Effect of confinement and viscosity ratio on the dynamics. The effect of the degree of confinement and viscosity ratio is syste. The process of lipid vesicle formation from double emulsions and the resulting lipid vesicles were imaged with a digital camera. We design a microcapillary device to generate dualcore double emulsion droplets, which contain two different inner aqueous drops labeled cores a and b inside an oil shell, as shown in fig. Kaplan p d, stone h a and weitz d a 2005 monodisperse double emulsions generated from a microcapillary device science 308 53741. This paper gives an overview of our recent work on the use of microfluidic devices to formulate double emulsions. Apr 22, 2005 double emulsions are highly structured fluids consisting of emulsion drops that contain smaller droplets inside.
The double emulsion device created by the lab works as follows. Development of novel drug formulation using micro uidic device. Weitz1 2 double emulsions are highly structured fluids consisting of emulsion drops that contain smaller droplets inside. We demonstrate high throughput synthesis of wow and owo double emulsions, without the need for timeconsuming surface treatment of the 3d printed micro. For the generation of monodisperse wow double emulsions, 20 cp. Controlled production of monodisperse double emulsions by. Controlled production of monodisperse double emulsions by two.
The aim of this study is to introduce a microcapillary devicebased micro. Results and discussion monodisperse double emulsions are generated with a glass microcapillary microfluidic device. Double wow emulsions consist in an inverse emulsion which is dispersed within a water continuous phase as direct emulsion droplets. Monodisperse double emulsions generated from a microcapillary device s. The double emulsions generated from microcapillary devices are very monodisperse as evidenced by the hexagonal close packing of the drops, illustrated by optical and. Double emulsions generated from a microcapillary device science 2005, 308, 537 541. Monodisperse coreshell microparticles size 100 to 250. Highthroughput production of micrometer sized double. Four reversible and reconfigurable structures for three. A monolithically threedimensional flowfocusing device for formation of singledouble emulsions in closedopen microfluidic systems. A microfluidic approach to encapsulate living cells in.
A reproducible approach to the assembly of microcapillaries for. Weitz science 308, 537 2005 micro and nanostructures through selfassembly guillaume tresset and shoji takeuchi. For producing waterinoilinwater wow dispersions, aqueous droplets ruptured at the upstream hydrophobic junction are enclosed within organic droplets formed at the downstream hydrophilic junction. A microfluidic device having both hydrophobic and hydrophilic components is exploited for production of multiplephase emulsions. The meniscus is stretched until a thin jet tapers from its tip due. The observations are compared with the predictions. Enhancedthroughput production of polymersomes using a. Microcapillary device monodisperse double emulsions generated from a. Double emulsion, microcapillary, microfluidics, emulsion. A reproducible approach to the assembly of microcapillaries.
Both the alginate microcapsule size and the thickness of alginate membrane can be easily controlled by modulating the dimensions of microfluidic device and the flow rates. Double emulsiontemplated monodisperse phospholipid vesicles. When the floor and ceiling are parallel, the quasistatic shape of the tongue is a circular pancake of radius r and curvature. Novel method for obtaining homogeneous giant vesicles from a monodisperse waterinoil emulsion prepared with a microfluidic device. Double emulsiontemplated monodisperse phospholipid. Monodisperse alginate microcapsules with oil core generated. Diatominspired microfluidic generation of tunable emulsions for macroporous silica by. Pdf monodisperse double emulsions generated from a.
Pdf synthetic membranes in microfluidic interfaces. A microcapillary device was used to make double emulsions in utada et al. For these experiments, the inner and middle phase flow rates were held constant and the carrier phase flow rate was modified to affect the shearing force during droplet generation. Gels made from soft microscale particles smoothly transition between the fluid and solid states, making them an ideal medium in which to create macroscopic structures with microscopic precision. Nov 12, 2008 the deformation and orientation of droplets during transient shear flow is studied in a counterrotating device using microscopy. Transition from squeezing to dripping in a microfluidic tshaped junction volume 595 m. Monodisperse double emulsion drops composed of an alginate droplet surrounded by a mineral oil drop dispersed in an aqueous cacl. This method utilises a threephase flow technique, where internal water droplets are.
Weitz1,2 double emulsions are highly structured fluids consisting of emulsion drops that contain smaller droplets inside. Oct 08, 2004 a microfluidic device having both hydrophobic and hydrophilic components is exploited for production of multiplephase emulsions. Droplets produced at each junction could have narrow. Designer emulsions using microfluidics an emulsion is a mixture of two immiscible liquids, where one liquid is dispersed in the form of small drops in another liquid that forms a continuous phase14.
Optical micrographs of the a formation of monodisperse owo double emulsions in the microfluidic device at flow rates of the inner oil, middle water, and outer oil phases of 0. The alginate microcapsules with oil cores are generated in a microcapillary microfluidic device using monodisperse oilinwaterinoil owo double emulsions as templates. Controlled formulation of monodisperse double emulsions in a. The production of compound particles is an important field. For the generation of monodisperse wow double emulsions. A modular microfluidic device via multimaterial 3d printing. Although decreases as r increases, it never becomes smaller than, meaning that the critical state for the thread is never reached. This device consists of four independent capillary microfluidic devices, operated in parallel. Droplets produced at each junction could have narrow size.
Using a microcapillary device, we fabricated double emulsions that contained a single internal droplet in a coreshell geometry. A microfluidic device having both hydrophobic and hydrophilic components. Generation of emulsion droplets and microbubbles in microfluidic. Second, we propose a similar fullglass device for generation of. All double emulsion generation processes were monitored with the microscope using a high speed camera. Jun 30, 2010 in the flow focusing technique, a liquid flow rate q is injected through a microcapillary to form a meniscus attached to its edge. The manual production of thousands of monodisperse nanoliterscale drops is shown in movie s4, where the parallel device of fig. Formation of monodisperse coreshell droplets with relatively thick shells. Microfluidic devices fabricated using stereolithography for. In contrast to bulk emulsification techniques, dropletbased microfluidics produce one droplet after the other, allowing precise tuning of the droplet diameters and the generation of highly monodisperse emulsions and double emulsions 2,3. In addition, monodisperse double emulsions with three different inner droplets can be generated in a device with three different inner channels.
Dropletbased microfluidics are a versatile tool to generate double emulsions, which is a droplet inside a droplet in a continuous phase. A monolithically threedimensional flowfocusing device for. This report presents a method of creating highly monodisperse single emulsions of high viscosity. Mar 19, 2018 monodisperse double emulsions generated from a microcapillary device. Microfluidic devices fabricated using stereolithography. Arrows indicate the flow direction of fluids and drops. New frontiers in aerosol delivery during mechanical ventilation. A uniform stream of double emulsions with multiple inner droplets nin1 was produced while operating in the jetting regime resulting from the high viscosity of the outer o2 phase as compared to that of the middle w phase 8. Double emulsion generation using a polydimethylsiloxane pdms. Oct, 2012 we report a parallelized capillary microfluidic device for enhanced production rate of monodisperse polymersomes. The deformation and orientation of droplets during transient shear flow is studied in a counterrotating device using microscopy. Monodisperse double emulsions generated from a microcapillary device article pdf available in science 3085721. Conclusions this work was supported by eps rc first grant no.
Double emulsions are highly structured fluids consisting of emulsion drops that contain smaller droplets inside. Designer emulsions using microfluidics ucla department of. Weitz1,2 double emulsions are highly structured fluids consisting of emulsion drops that contain smaller droplets. We show that threedimensional devices fabricated using stereolithography can generate monodisperse double emulsions and enable precision control of the sizes and number of encapsulated droplets.
Microfluidic method for creating monodisperse viscous. The aim of this paper is to explore the basic rules that govern the stability of double emulsions. A monolithically threedimensional flowfocusing device. Jun 19, 2014 creating droplets with viscous media in microfluidic devices is extremely problematic due to the early onset of jetting.
Weitzmonodisperse double emulsions generated from a microcapillary device. Forced generation of simple and double emulsions in all. Using a microcapillary device, we fabricated double emulsions that contained a single internal droplet in a core. A modular microfluidic device via multimaterial 3d. Double emulsions have a high potential for applicability within the commercial field, because the middle liquid shields the inner liquid from the outer and so allows for effective. Monodisperse double emulsions generated with a microcapillary. H a group of double emulsion drops, each containing a single inner drop. A new assembly method and device for microfluidic double emulsion. Published 15 september 2006 2006 iop publishing ltd journal of micromechanics and microengineering, volume 16, number 11. Microfluidic method for creating monodisperse viscous single. Key issues in the controlled encapsulation of highly monodisperse drops include.
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